Journal: PLoS ONE

Article Title: Inhibition of ERβ Induces Resistance to Cisplatin by Enhancing Rad51–Mediated DNA Repair in Human Medulloblastoma Cell Lines

doi: 10.1371/journal.pone.0033867

Figure Lengend Snippet: Panel A: Fluorescent images showing nuclear morphology following labeling of DNA by fluorescent dye 4′,6-diamidino-2-phenylindole (DAPI). Exponentially growing monolayer cultures of Daoy cells (10%FBS) were treated with cisplatin (1 µg/ml) or with cisplatin + ICI182,780 (10 µM) for 48 hours. The images were taken with Nikon Eclipse 400 upright fluorescent microscope equipped with the motorized Z-axis, EXI-Aqua camera and deconvolution software (SlideBook5). Rectangles indicate magnified area containing cells in mitosis (asterix); and cells with damaged nuclei (arrowhead). Note that abundant presence of micronuclei (arrow) and nuclear fragmentation in cisplatin, and much less of the nuclear damage in cells treated by cisplatin+ICI182,780. Original magnification ×20. Panel B: Daoy cell viability evaluated by ViaCount and TUNEL assays. Both assays were adopted for the use with the GUAVA easyCyte 8HT flowcytometer (Millipore). The Guava/Express Plus and Guava/ViaCount software were used for data analysis and quantification according to the manufacturer recommendations (Millipore).

Article Snippet: The images were visualized with the Nikon Eclipse E400 upright fluorescence microscope equipped with EXI aqua camera (Qimaging), motorized Z-axis, and SlideBook5 acquisition/deconvolution software (Intelligent Imaging Innovations, Inc., Denver, CO).

Techniques: Labeling, Microscopy, Software, TUNEL Assay